中国临床药理学与治疗学2012，Vol.17Issue(5)：491-496,6.
皖南蝮蛇毒抑瘤组分Ⅰ对人胃癌细胞SGC-7901荷瘤鼠体内抑制作用的研究
In vivo tumor-inhibitory effects of Agkistrodon halys venom antitumor component Ⅰ on tumor- bearing mice with human gastric cancer cell SGC-7901
摘要
Abstract
AIM: To investigate the in vivo tumor-inhibitory effects of snake venom antitu-tnor component KAHVAC-I) from Agkistrodon halys living in south Anhui province on the tumor-bearing mice with human gastric cancer cell SGC-7901 and activities of the cell proliferation as well as its mechanisms. METHODS: Cell suspension of 0. 1 mL was obtained from the well-grown cell SGC-7901 by cell count of 1 X 107/mL and incubated subcutaneously into the nude mice via right axillary fossa. After tumor formation, the mice were randomized into groups of model controls, positive paclitaxiel controls, AHVAC-I of low, medium and low dosage in which the animals were managed with AHVAC-I every other day for 5 consecutive times. The tumor growth and its sizes were observed and measured for mapping the growth curve and calculating the inhibitory rate. HE staining was performed to examine the morpho- logic change of tumors, and streptavidin-peroxi-dase (SP) immunohistochemical technique was used to detect the proliferation index of Ki-67 and TUNEL method was for examination of the apoptotic index. RESULTS: AHVAC-I at dose of 1. 0 mg/kg produced inhibitory effects on SGC-7901 with inhibition rate of 42.2%, and inhibitory effects were significant at dose of 3.0 mg/kg with a rate of 78. 5%. Ki-67 protein was reduced in the positive cells of treatment groups, leading to increased apoptosis of tumor cells. CONCLUSION; AHVAC-I can effectively inhibit the in vivo growth of gastric cell SGC-7901 in nude mice, and the possible mechanisms may be associated with the induced apoptosis and suppressed proliferation of the gastric tumor cells.关键词
皖南蝮蛇毒抑瘤组分1/胃癌/裸小鼠/抑制/增殖/凋亡Key words
AHVAC-I/ Gastric cancer/Nude mice/ Inhibition/ Proliferation/ Apoptosis分类
医药卫生引用本文复制引用
卢林明,周珏,张根葆..皖南蝮蛇毒抑瘤组分Ⅰ对人胃癌细胞SGC-7901荷瘤鼠体内抑制作用的研究[J].中国临床药理学与治疗学,2012,17(5):491-496,6.基金项目
安徽省科技厅基金资助项目(10021303009) （10021303009）
芜湖市科技局科研基金项目(09卫生类1-1) （09卫生类1-1）
