中国临床药理学与治疗学2012，Vol.17Issue(5)：497-501,5.
低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响
Effect of oligomeric grape seed proanthocyanidins on prevention isoproterenol induced cardiomyocytes hypertrophy
摘要
Abstract
AIM: To investigate the effect of oligomeric grape seed proanthocyanidins (GSP) on neonatal cardiomyocytes hypertrophy induced by isoproterenol (Iso). METHODS: Hypertrophy in cultured neonatal rat cardiac myocytes was induced by Iso. Total protein content was measured by BCA method; reactive oxygen species (ROS) were detected by flow cytometry; colorimetric method was employed to measured MDA content and SOD antivity. RESULTS-1 X 10~5 mol/L Iso can activate f)-AR completely and induce cardiomyocytes hypertrophy markedly. GSP therapy potently inhibited the increase of protein content, ROS generation and MDA content, inhibited the decrease of SOD activity with a dose-dependent manner. CONCLUSION: GSP possess protective effect against Iso induced cardiomyocytes hypertrophy, this may be related to reducing the ROS generation and oxidative stress level, at least in part.关键词
低聚葡萄籽原花青素/异丙肾上腺素/心肌细胞肥大/氧自由基/丙二醛/超氧化物歧化酶Key words
Oligomeric grape seed proanthocyanidins/ Isoproterenol/ Cardiomyocytes hypertrophy s Reactive oxygen species/ Malondialdehyde (MDA)/ Superoxide dismutase (SOD)分类
医药卫生引用本文复制引用
高杉,黄春霞,黄玲玲,俞婷婷,汪星辉,张野..低聚葡萄籽原花青素对异丙肾上腺素诱导心肌细胞肥大的影响[J].中国临床药理学与治疗学,2012,17(5):497-501,5.基金项目
教育部高等学校博士学科点基金(20103420120002) （20103420120002）
国家自然科学基金(81073088) （81073088）
安徽医科大学博士启动基金(XJ200709) （XJ200709）
