中国临床药理学与治疗学2012，Vol.17Issue(5)：543-548,6.
焦磷酸测序技术检测SLCO181基因多态性方法的建立
Development of pyrosequencing method for detection of SLCO1B1 polymorphisms
摘要
Abstract
AIM: To establish a pyrosequencing based method for detection SLCO1B1 A388G and T521C polymorphisms and to determine the frequency of these polymorphisms in healthy Chinese. METHODS: After preparation of gD-NA from blood of 300 subjects, the target frag- ments were amplified by PCR, polymorphisms were detected on PyroMark ID by pyrosequencing technology. The reliability of pyrosequencing methods were validated by repeat tests and Sanger sequencing. RESULTS: We established a new pyrosequencing method to detect the SL- C01B1 A388G and T521C polymorphisms polymorphisms in healthy Chinese. The detection rate and repetition rate were both 100%. The frequencies of 388A, 388G, 521T and 521C al-leles were 28%, 72%, 89. 5% and 10. 5%, respectively. Genotype frequencies match the Hardy-Weinberg equilibrium. CONCLUSION- These pyrosequencing assays to detect SLCO1B1 poly- morphisms are proved to be a rapid, accurate and high-throughput alternative to conventional methods, and it can be a preferred option in research and clinical application.关键词
焦磷酸测序/SLCO1B1/OATP1B1/单核苷酸多态性分类
医药卫生引用本文复制引用
万子睿,谢海棠,郭栋,胡东莉,王丽萍,王果..焦磷酸测序技术检测SLCO181基因多态性方法的建立[J].中国临床药理学与治疗学,2012,17(5):543-548,6.基金项目
国家自然科学基金项目(81072706,81173134) （81072706,81173134）
高等学校博士学科点专项科研基金资助课题(20090162120024) （20090162120024）
湖南省科技计划项目(2009JT3020) （2009JT3020）
