中国美容医学2012，Vol.21Issue(5)：759-761,3.
黑木耳多糖对ICR小鼠皮肤光老化的保护作用
Protective effects of Auricularia auricular polysaccharide on skin photoaging in ICR mice
摘要
Abstract
Objective To study the protective effects of Auricularia auricular polysaccharide (AAP)on photoaged ICR mice skin. Methods Fifty healthy female ICR mice were divided into five groups (n = 10) randomly: normal group(A), model control group (B),at dose of 50 mg/kg.d group (C), at dose of 100 mg/kg.d group (D), at dose of 200 mg/kg.d group (E). To establish skin photoaging mice models by irradiated UVA and UVB three times weekly (Monday, Wednesday and Friday).ln the first week the time of UV exposure was 1 hour each time, then increased by 1 hour per week until week 5, and maintained the time until the 14th week. Before every UV irradiation, the AAP were orally administered with different dose to treated mice groups respectively. At the end of the experiment,the superoxide dismutase (SOD) and glutathione peroxidase (GSH-PX) activities, malondialdehyde (MDA) content in the skin were detected by assay kit.The skin tissue was observed by HE staining histological sections. Results Compared with the normal group, the activities of SOD and GSH-PX were decreased significantly (P<0.05),and the content of the MDA was increased significantly (P<0.05) in the model control group. Compared with the model control group, the activities of SOD and GSH-PX in group C, D and E were increased significantly (P<0.05),and the content of MDA was reduced significantly (P<0.05) in every AAP treatment group.The model control group appeared photoaging features, such as scale,brown-yellow color,coarse,thickened,deep wrinkles and poor flexibility. While compared with the model control group,both the skin appearance and histological change of AAP treatment groups were improved significantly. Conclusion AAP has protective effect on the skin photoaging in ICR mice via increasing the capacity of anti-oxidation.关键词
光老化/黑木耳多糖/ICR小鼠Key words
photoaging/ Auricularia auricular polysaccharide/ ICR mice分类
医药卫生引用本文复制引用
易清玲,曾维惠,刘彦婷,徐磊..黑木耳多糖对ICR小鼠皮肤光老化的保护作用[J].中国美容医学,2012,21(5):759-761,3.基金项目
中华医学会皮肤性病学分会 昆明滇虹药业有限公司薇诺娜皮肤病学研究基金 （）
