中国美容医学2012，Vol.21Issue(6)：941-944,4.
不同支架材料体外构建组织工程软骨的研究
Study on constructing tissue-engineered cartilage in different scaffolds in vitro
摘要
Abstract
Objective Try to construct the tissue -engineered cartilage and selection a better scaffold for the constructing. Methods Treating the first generation chondrocytes as the seeds which was separated from the SD rat. Through the adjusting the concentration of chondrocytes ,we can make the complex which is composed by cancellous bone matrix (BMG) / biological fibrin glue, chitosan/gelatin (Cs-gel) and chondrocytes. In different culture time, HE, toluidine blue (TB),Massons triad colour,immunohistochemistry, immunofluorescence and scanning electron microscope were used to test the complex. Results After co-culturing two weeks, we can certify the BMG/ biological fibrin glue is precendecer than Cs/gel.Concretly, the chondrocytes is uniform in BMG/biological fibrin glue,but not adqulis in Cs/gel. aggrecan and collagen Ⅱ is obviously in testing.But the longer the co-culturing, all tests display that special aggrean and collagen Ⅱ about chondrocytes are obvious at eight weeks the latter is better than the former. Conclusion Succeeding in constructing the tissue-engineered cartilage. In early culture,the growth,proliferation and secretion of chondrocytes in BMG/ biological fibrin glue was better.But Cs/gel have significallly advantage in longer co-culturing in vitro.关键词
软骨细胞/组织工程/壳聚糖/明胶/BMG/生物蛋白胶Key words
chondrocytes/tissue-engineered cartilage/chitosan/gelatin/cancellous bone matrix (BMG)/ biological fibrin glue分类
医药卫生引用本文复制引用
葛平,王正辉,杨壮群,王瑞,温绣蔺..不同支架材料体外构建组织工程软骨的研究[J].中国美容医学,2012,21(6):941-944,4.基金项目
国家自然科学基金资助项目(81000416) （81000416）
2011年中央高校基本科研业务费专项资金(西安交通大学国际合作类,交叉学科类) （西安交通大学国际合作类,交叉学科类）
