中国免疫学杂志2012，Vol.28Issue(7)：589-593,5.DOI:10.3969/j.issn.1000-484X.2012.07.003
重组金黄色葡萄球菌肠毒素C3生物学活性的初步研究
Expression and preliminary bioactivity analysis of the recombinant Staphylococcus aureus enterotoxin C3
摘要
Abstract
Objective:To express the recombinant Staphylococcus aureus enterotoxin C3 (rSEC3) and preliminary investigate its activity. Methods:E. coli BL21 containing the recombinant plasmids pET-32a( + )-SEC3 was expressed and purified. Then the human PBMCs was stimulated with different concentration of rSEC3 at 0. 1,0. 5,1,5,10,50,100μg/ml and the promoting proliferation activity was detected by MTT method. Last, the tumor-cytotoxicity effects of Hela and KYSE were examined after being induced 48 h. Results;The recombinant Staphylococcus aureus enterotoxin C3 was expressed efficiently in E. coli BL21. Compared with blank control, 0. 1-100μg/ml of rSEC3 could significantly promote proliferation of human PBMCs (P ＜0. 05) . 1μg/ml of rSEC3 was the most efficient dose (t = 113.851 ,P =0.000) and showed a similar effect with 100 μg/ml PHA(r = 0. 693 ,P = 0. 527) . After cultured 48 h, rSEC3 at 0. 5, 1, 5 μg/ml had a strong depressant effect on Hela by activating PBMC compared with blank control (P ＜0. 05). The inhibition rates were (70.44 ± 9. 27) % ,(93.65 ±8.05)% ,and (103.40 ±6. 65) %. Furthermore, the rSEC3 also enhanced the killing activity to KYSE cells (P ＜0.05) , and the inhibition rates were (28. 10 ± 2. 72)% , (46. 62 ±4. 17)% ,and(19.35 ± 3.05)% , respectively. Conclusion:The recombinant protein rSEC3 has activity on human PBMCs proliferation, and it can enhance PBMCs killing effect on tumor cells.关键词
金黄色葡萄球菌/肠毒素/表达/生物学活性Key words
Staphylococcus aureus/Enterotoxin/Expression/Bioactivity分类
医药卫生引用本文复制引用
李先平,王敏,武婷,梅城,曹虹..重组金黄色葡萄球菌肠毒素C3生物学活性的初步研究[J].中国免疫学杂志,2012,28(7):589-593,5.基金项目
本文为湖南省自然科学基金(06JJ4051) （06JJ4051）
