中国免疫学杂志2012，Vol.28Issue(7)：645-647,3.DOI:10.3969/j.issn.1000-484X.2012.07.016
调节性T细胞及其分泌的细胞因子在再生障碍性贫血中的意义
The significance of regulatory T cells and cytokines secreted by regulatory T cells in aplastic anemia
摘要
Abstract
Objective; To investigate the pathogenes is -related immunological mechanisms of T cell subsets in aplastic anemia by dectecting ratio of T cell subsets and the level of cytokines secreted by Treg cells. Methods:Basing on clinical diagnostic criteria, the peripheral blood samples of aplastic anemia were gathered from the initial issuance, the hematopoietic recovery and normal controls. The proportion of CD4+ ,CD8 + ,NK,NKT,Treg cells were counted by FACS respectively. The Treg cells of samples were separated by immunomagnetic beads and observed by stimulating factors. The supernatants were then collected after 24 or 48 hours. Enzyme-linked immunosorbent assay (ELISA) was applied to examine the expression of IL-10,IL-35 and TGF-p secreted by Treg cells. ResultS;The proportion of CD4+ , Treg cells in the initial issuance was the lowest among the groups. The result was statistically significant (P＜ 0.05) that the proportion of CD8 + T cells was significantly higher in patients with aplastic anemia. There was no statistical difference in NK,NKT cells between aplastic anemia and normal controls(P ＞0. 05) . The culture supernatant in ELISA showed that the expression of IL-10,IL-35 and TGF-β was significantly lower (P＜0.05) in the initial cases compared to the normal controls. Conlusioil: Dysimmunity of T cells is a significant link in the aplastic anemia pathogenesis. Treg cells may play a positive regulatory role in the process of hematopoietic function recovery of aplastic anemia patients.关键词
再生障碍性贫血/T细胞亚群/调节性T细胞Key words
Aplastic anemia/T cell subsets/Regulatory T cells分类
医药卫生引用本文复制引用
黄国清,刘秋爽,程永红,黎阳,郭敏,郭强忠,金鑫,李文郎..调节性T细胞及其分泌的细胞因子在再生障碍性贫血中的意义[J].中国免疫学杂志,2012,28(7):645-647,3.基金项目
本文为深圳市科技计划项目(No.201103059) （No.201103059）
