农学学报2012，Vol.2Issue(6)：27-30,48,5.
黑粒小麦‘冬黑10号’的细胞学和麦谷蛋白组成分析
Analysis of Cytology and Glutenin Composition in Black Kernel Wheat ＇Donghei 10＇
摘要
Abstract
Shanxi Province was a important producing area of quality wheat in China, had been developed and approved six black kernel wheat varieties by Shanxi Crop Variety Approval Committee, however, their chromosome and protein constitutions of these cuhivars were less reported. Understanding its genetic composition would provided useful information for breeding. In this paper, chromosome configuration of black kernel wheat Donghei 10 was characterized by chromosome C-banding, genomic in situ hybridization, chromosome pairing configuration and glutenin subunit analysis. The results showed that the chromosomes numbered 42 （2n= 42） in its root tip-cells, chromosome C-banding patterns no significant changes from Chinese Spring, in the genomic in situ hybridization were not detected Leymus secalinus chromosomes. The mean chromosome pairing configuration was 2n= 21 II at PMC M 1, indicating Donghei 10 with good stability of cytology, and F1 hybrids with Chinese spring PMC M I chromosome configuration of 21 II showed to be hexaploid common wheat. SDS-PAGE analysis revealed that HMW-GS composed of Null/7 ＋ 9/5 ＋ 10, in addition to LMW-GS differing from that of Chinese Spring.关键词
黑粒小麦/C-分带/原位杂交/高分子量麦谷蛋白亚基/低分子量麦谷蛋白亚基Key words
Black Kernel Wheat/C-banding/GISH/' HMW-GS/LMW-GS分类
农业科技引用本文复制引用
郭明慧,裴自友,温辉芹,张立生,程天灵,李雪..黑粒小麦‘冬黑10号’的细胞学和麦谷蛋白组成分析[J].农学学报,2012,2(6):27-30,48,5.基金项目
山西省回国留学人员科研资助项目“小麦优质抗病分子标记设计育种” （）
山西省科技攻关项目“山西中部高产优质抗逆小麦新品种选育” （）
山西省农业科学院科研项目“小麦优质高产广适多抗系列新品种选育”（1lyzgc042）. （）
