中国农业大学学报2012，Vol.17Issue(3)：28-33,6.
獐茅耐盐基因SOS1的克隆及序列分析
Cloning and sequence analysis of plasma membrane Na~＋/H~＋ antiporter gene SOS1from Aeluropus littoralis
摘要
Abstract
A plasma membrane Na＋/H＋ antiporter gene（AlSOS1,JN936862） was isolated from Aeluropus littoralis（Gouan） Parl,a graminaceous halophyte.Southern blot analysis indicated that there was only one copy AlSOS1 gene in the A.littoralis genome.The AlSOS1 sequence contained 3 641 bp with an open read frame of 3 420 bp and the deduced transcripts encoding 1 139 amino acids shared a relative high homology with that of reed（82%） and rice（77%）,while a low homology with that of dicot plants as Arabidopsis（58%）.A phylogenetic analysis of a number of Na＋/H＋ antiporter was shown AlSOS1 formed a clade with the most closely related plant SOS1 homolog,which was distinct from the cluster of vacuolar Na＋/H＋ antiporters.Through hydrophobicity plot analysis,the AlSOS1 protein was predicted containing 12 transmembrane domains in the N-terminal part and a long hydrophilic cytoplasmic tail in the C-terminal part.In a word,the AlSOS1 cDNA was first cloned completely,and suggested the potential utilization for manipulating monocot crops for cultivation under high-salt conditions.关键词
獐茅/耐盐基因/质膜Na＋/H＋逆向转运蛋白基因（SOS1）/cDNA克隆/序列分析Key words
Aeluropus littoralis/plasma membrane Na+/H+ antiporter gene SOS1/cDNA cloning/sequence analysis分类
农业科技引用本文复制引用
王鹤,张高华,王旭达,丰明,李怀梅,李树英..獐茅耐盐基因SOS1的克隆及序列分析[J].中国农业大学学报,2012,17(3):28-33,6.基金项目
辽宁省博士科研启动基金 （）
