中国农业大学学报2012，Vol.17Issue(3)：63-68,6.
珠眉海棠水通道蛋白MzPIP1;1基因的克隆与表达分析
Cloning and expression analysis of MzPIP1;1 gene from Malus zumi Mats
摘要
Abstract
The aquaporins play a central role in water transportation in plant.The plant under salt stress can maintain water balance through regulating the expression and activity of aquaporins.Malus zumi is an apple rootstock that can survive 0.6% NaCl.The full-length cDNA sequence of MzPIP1;1 gene was isolated from a cDNA library of Malus zumi under salt stress by microarray analysis.The full-length cDNA of MzPIP1;1 was 1 135 bp.Its open reading frame of 870 bp encoded a protein of 289 amino acids.The 5′-UTR and the 3′-UTR were 91 and 174 bp respectively.Multiple sequence alignment assigned MzPIP1;1 to PIP1（Plasma membrane Intrinsic Protein 1） subfamily,which had the high sequence identity at the amino acids level（more than 80%） with PIP1s from other 4 plant species.The MzPIP1;1 was localized in plasma membrane and induced by the stresses of high-salinity and low temperature,which might be related to the potential role of MzPIP1;1 in salt tolerance of Malus zumi Mats.关键词
珠眉海棠/MzPIP1/1/盐胁迫/表达特征Key words
Malus zumi Mats/salt stress/MzPIP1/1/expression pattern分类
农业科技引用本文复制引用
李擎天,赵宇,周绮云,黄黎芳,蒋玉妆,陈浩,孔瑾..珠眉海棠水通道蛋白MzPIP1;1基因的克隆与表达分析[J].中国农业大学学报,2012,17(3):63-68,6.基金项目
国家自然科学基金 （）
北京市自然科学基金 （）
