中国农业大学学报2012，Vol.17Issue(3)：127-130,4.
反相高效液相色谱法同时检测多种乳清蛋白成分
Determination of components of whey protein by reversed-phase HPLC method
摘要
Abstract
The aim of this study was the rapid determination of α-lactalbumin（α-La）,β-lactoglobulin（β-Lg）,Lactoferrin（Lf）.After removing fat from whey protein by centrifugation and precipitating caseins by adjusting pH,the whey contents were detected by Dikma Proteonavi Chromatographic column with 0.1%（volume fraction） TFA solution and acetonitrile solution by 0.5 mL/min flow rate,gradient elutio and column temperature was stetted at 35 ℃.Detection was carried out at the wavelength 280 nm.The results illustrate that the separation of α-La,β-Lg and Lf is available.The linear realationship of standard samples is good,and the recovery of the standard samples is in the normal range.The method which is simple,fast and accurate can determine the content of α-La,β-Lg and Lf of breast milk,milk and transgenic milk.关键词
乳品/α-乳白蛋白/β-乳球蛋白/乳铁蛋白/高效液相色谱Key words
dairy/α-lactalbumin/β-lactoglobulin/Lactoferrin/HPLC分类
轻工纺织引用本文复制引用
朱宏,邢志恩,戴蕴青,陈敏..反相高效液相色谱法同时检测多种乳清蛋白成分[J].中国农业大学学报,2012,17(3):127-130,4.基金项目
转基因生物品种培育重大专项资助 （）
