中国农业科技导报2012，Vol.14Issue(2)：95-100,6.DOI:10.3969/j.issn.1008-0864.2012.02.15
重离子诱变选育聚β-羟基丁酸酯高产菌株研究
Studies on Selecting High-yield Poly-β-hydroxybutyrate Producing Strain by Heavy-ion Irradiation
摘要
Abstract
As a new biodegradable polymer materials, Poly-|J-riydroxybutyrate ( PHB) has attracted wide attention from people day by day. This study adopts heavy-ion mutation breeding technology, using I2C ion beams to mutate the PHB producing Strain-Bacillus Z-3, then screen a PHB high yielding strain Al 1 from a mount of mutant strains. Its PHB output can reach 1.404 g/L. This is 1. 33 times as compared with the original strain. The results indicate that heavy-ion mutation breeding technology has good mutagenic effects. It is an effective method and way to microbial mutation breeding.关键词
聚β-羟基丁酸/重离子/诱变/选育/高产菌株Key words
poly-p-hydroxybutyrate/heavy ion/mutagenesis/selective breeding/high-yield strain分类
生物科学引用本文复制引用
张楠,张昺林,王婉如,徐俊泉,张东明,薛林贵..重离子诱变选育聚β-羟基丁酸酯高产菌株研究[J].中国农业科技导报,2012,14(2):95-100,6.基金项目
甘肃省科技支撑计划项目(090NKCA079) （090NKCA079）
国家自然科学基金项目(30870384) （30870384）
铁道部科技研究开发计划课题(2010Z001-E)资助. （2010Z001-E）
