中国农业科学2012，Vol.45Issue(6)：1106-1115,10.DOI:10.3864/j.issn.0578-1752.2012.06.008
胞囊线虫侵染后小粒黑豆抑制消减杂交cDNA文库的构建及EST分析
Construction of SSH-cDNA Libraries and EST Analysis in Roots of Xiaoli Black Bean in Response to Heterodera glycines Parasitization
摘要
Abstract
[ Objective] The objective of this study is to research into the early stage root gene expression profile of G max induced by H. Glycines and to explore the resistant mechanism to the pathogen at molecular level. [ Method ] SSH-cDNA libraries enriched with differentially expressed ESTs were constructed from SCN-challenged root tissues at pre-penetration and early infection stages from Xiaoli black bean (G max) by suppression subtractive hybridization and reverse Northern blot. [ Result ] One hundred and sixty-six unique ESTs were identified and analyzed with Blastx and Blastn by comparing sequences in the GenBank. One hundred and ninteen unigenes accounted for 83% of all the unigenes showed high homology with the function known genes or proteins. The function-known ESTs were annotated into functional categories including signal recognition and transduction, energy and material metabolism, stress responses, transcriptional regulation, protein synthesis and/or modification, transport functions, cellular architecture. [Conclusion] Discreet gene tag clusters primarily including catalase, ubiquicin, chitinase, lipoxygenase, aquaporin, ripening related protein, metallothionein, plasma membrane intrinsic protein, cytochrome P450, glyceraldehyde-3-phosphate dehydrogenase were abundant in the SCN-infected roots. It is speculated that those genes played an important function in the incompatible interaction between Xiaoli black bean and H. Glycines.关键词
大豆胞囊线虫/大豆/抑制消减杂交/表达序列标签Key words
Eeterodera glycines/Glycine max/suppression subtractive hybridization/EST引用本文复制引用
王芳,段玉玺,陈立杰,王媛媛,朱晓峰,王东..胞囊线虫侵染后小粒黑豆抑制消减杂交cDNA文库的构建及EST分析[J].中国农业科学,2012,45(6):1106-1115,10.基金项目
国家自然科学基金(31171569)、国家现代农业产业技术体系岗位科学家专项(CARS-04-PS13) （31171569）
