中国农业科学2012，Vol.45Issue(6)：1183-1190,8.DOI:10.3864/j.issn.0578-1752.2012.06.017
关中奶山羊Prm1基因的克隆及真核表达
Cloning of Dairy Goat Prm1 Gene CDS and Construction of the Eukaryotic Expression Vector
摘要
Abstract
[objective] The objective of the experiment is to study the Prml gene of dairy goat, and for further research on dairy goat spermatogenesis. [Method] The CDS of Prml gene was cloned by PCR from Guanzhong dairy goat testis tissue. The obtained sequence was constructed into the eukaryotic expression vector pIRES-GFP, and then transfected to GC1 cell line and human umbilical cord cells. PCR and immunohistochemistry were used to test the expression of Prml at different ages of dairy goat testis. [Result] In dairy goats, the expression of Prml gene was high and specific in adult testis, located only in the head of sperm. The CDS of Prml gene of Guanzhong dairy goat was amplified and compared with the sequences of other species. The results showed that they shared a high homology, being 97.4% between dairy goats and bovine. The Prml gene was overexpressed in GC1 cells and human umbilical cord cells to confirm its function. [Conclusion] The complete CDS of dairy goat Prml gene and its expression patterns were obtained.关键词
关中奶山羊/Prm1基因/基因克隆/表达规律Key words
Guanzhong dairy goat/Prml gene/gene cloning/expression pattern引用本文复制引用
李明昭,刘超,孙军伟,朱海鲸,曹晖,吕晓,仇普斌,华进联..关中奶山羊Prm1基因的克隆及真核表达[J].中国农业科学,2012,45(6):1183-1190,8.基金项目
国家自然科学基金(30200137)、教育部重点科研项目(109148)、中国博士后基金(20080431253)、中国博士后科学基金特别资助项目(200801438)、留学回国人员科研启动费(14110101)、陕西省科技攻关(2008K02-05)、西北农林科技大学青年学术骨干支持计划(01140301) （30200137）
