中国农业科学2012，Vol.45Issue(6)：1199-1205,7.DOI:10.3864/j.issn.0578-1752.2012.06.019
奶牛乳腺炎源大肠杆菌中耶尔森菌强毒力岛相关基因的检测及序列分析
Detection of High Pathogenicity Islands in Escherichia coli Isolated from Clinical and Subclinical Bovine Mastitis in Some Areas of China
摘要
Abstract
[Objective] The objective of the experiment is to investigate the present distribution of high pathogenicity island (HPI) in E. coli isolated from cows with clinical and subclinical mastitis in China. [Method] Milk samples from 1 260 cows with clinical and subclinical mastitis in different farms and areas were used to screen Gram-negative bacteria by MacConkey agar. One hundred and ninety E. coli isolates was further identified from the Gram-negative bacteria by microbiological tests. Total DNA extraction from E. coli was used to detect HPI genes by PCR. [ Result] The PCR results showed that 50 out of 190 isolates carried the irp2 gene, 36 isolates were positive for fyuA gene and 32 for intB genes. Sequence analysis of randomly selected PCR products showed that homology of genes irp2, fyuA, and asntRNAintB were above 97.1%, 98.2%, and 97.2% identical to the published sequences. HPI* isolates were associated with fyuA (72%) and asn tRNA locus (64%). [Conclusion] Yersinia HPI was widely distributed in the E. coli isolates from cows with clinical and subclinical mastitis in China. The prevalence of HPI is associated withspecial serotypes in E.coli from cows with clinical and subclinical mastitis.关键词
奶牛乳腺炎/大肠杆菌/HPI毒力岛/O血清型Key words
bovine mastitis/Escherichia coli/high pathogenicity island (HPI)/O serogroups引用本文复制引用
徐继英,杨志强,陈化琦,刘俊林,邢娟,李建喜,李宏胜..奶牛乳腺炎源大肠杆菌中耶尔森菌强毒力岛相关基因的检测及序列分析[J].中国农业科学,2012,45(6):1199-1205,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(BRF070304) （BRF070304）
甘肃省自然科学基金项目(3ZS061-A25-072) （3ZS061-A25-072）
