中国农业科学2012，Vol.45Issue(6)：1226-1234,9.DOI:10.3864/j.issn.0578-1752.2012.06.022
小麦逆境胁迫相关基因Ta14S的克隆及表达分析
Cloning and Expression Analysis of A Stress-related Ta14S Gene from Wheat
摘要
Abstract
[Objective] In this study, the objective is to clone the stress resistance-related genes, and analyze the expression pattern of those genes which aim to investigate the underlying molecular mechanism of stress resistance and provide candidate genes for stress-resistance plant breeding. [Method] Based on the analysis of up-regulated EST obtained by cDNA chip, a full-length cDNA sequence was cloned from wheat through rapid amplification of cDNA ends (RACE) method and proper bioinformatics softwares were applied for characterizing the cloned genes and the deduced proteins. The gene expression characters were analyzed by the real time PCR. [Result] The full-length cDNA sequence designated as Tal4S from wheat was 1 056 bp in length, contains a 792 bp open reading frame (ORF) encoding a 263 amino acid proteins, with 11 bp in the 5' UTR and 253 bp in the 3' UTR. Similarity alignment of plant 14-3-3 proteins by DNAMAN revealed four high conserved domains. These domains were a potential substrate for protein kinase C, annexin-like domain, domain to bind transcription factor and nuclear export signal peptide, respectively. This result suggests that the Tal4S belongs to the family of 14-3-3. Real time PCR analysis revealed that the expression level of Tal4S was the highest in root than in leave and stem. The expression of Tal4S was steadily up-regulated in any of the time points under PEG and low temperature stress and the relative expression level was significantly higher than those of control within 6 h of ABAand low temperature stress treatments. This result suggests that Tal4S may be involved in stress resistance-related response of ABA signaling pathways in wheat roots. [Conclusion 1A full-length cDNA of TaI4S was cloned from wheat and the typical protein interact binding domains were found in the deduced proteins. The expression patterns of Tal4S gene under drought, high and low temperature and ABA treatments showed that Tal 4S might play an important regulation role under stress in wheat. The results provided important information for further studies on molecular regulation mechanism underlying abiotic stress resistance in wheat关键词
小麦/14-3-3蛋白/逆境胁迫/表达Key words
wheat (Triticum aestivum)/14-3-3 protein/stress/expression引用本文复制引用
任江萍,刘海伦,王新国,牛洪斌,李永春,王翔,陈新,尹钧..小麦逆境胁迫相关基因Ta14S的克隆及表达分析[J].中国农业科学,2012,45(6):1226-1234,9.基金项目
国家转基因生物新品种培育重大专项(2009ZX08002-011B) （2009ZX08002-011B）
