中国农业科学2012，Vol.45Issue(10)：1972-1981,10.DOI:10.3864/j.issn.0578-1752.2012.10.010
草莓AP1同源基因的克隆、表达及启动子分析
Cloning, Expression and Promoter Analysis of AP1 Homologous Gene from Strawberry (Fragaria ×ananassa)
摘要
Abstract
[Objective] The aim of this study was to isolate the AP1 homologous gene from strawberry (Fragaria譨nanassa), analyze its expression level in different tissues, organs and at different growth stages of flowers and elucidate the role of it in the floral development progress of strawberry[ Method ]The fragment of an AP1 homologous gene was amplified from young leaves and flower buds of strawberry with the degenerate primers designed according to the conserved sequences of AP1 homologous genes in the other species. The full-length cDNA sequence of this gene was obtained with RACE technique. Real time RT-PCR was used to analyze the expression patterns of this gene in different tissues, organs and at different growth stages of flowering of strawberry. A promoter sequence was isolated with chromosome walking methods. [Result] An AP1 homologous gene in strawberry was cloned from cultivar 'Huaji' and it was designated as FaAP1. The CDS length of FaAP1 is 735 bp and encodes a predicted protein of 245 amino acid residues. Its amino acid sequence shows a highest homology with the AP1-1 of Rosa, up to 92%, and also has a homology of 64.00% when compared with the AtAP1 of Arabidopsis thaliana. FaAPl is a MK.C type MADS-box gene for its amino acid sequence contained the typical domains of MADS-box, 1-region, K-box and C-terminal. The results of real time RT-PCR showed that the expression patterns of FaAP1 changed in different tissues, organs and flowers of different growth stages. In addition to the TATA/CAAT-box, its promoter also contained some specific regulatory elements. [ Conclusion ] FaAP1, an AP1 homologous gene in strawberry, was isolated and it may play an important role in the progress of flowering in strawberry.关键词
草莓/AP1同源基因/分离/表达/启动子Key words
strawberry/ AP1 homologue/ isolation/ expression/ promoter引用本文复制引用
邹冬梅,刘月学,张志宏,李贺,马跃,代红艳..草莓AP1同源基因的克隆、表达及启动子分析[J].中国农业科学,2012,45(10):1972-1981,10.基金项目
国家自然科学基金项目(30871689、31101524)、辽宁省教育厅创新团队项目(LT2010094)、沈阳农业大学青年教师科研基金资助项目(20070201) （30871689、31101524）
