中国农业科学2012，Vol.45Issue(10)：2002-2011,10.DOI:10.3864/j.issn.0578-1752.2012.10.013
填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度
Fatty Acids Deposition and FAS mRNA Expression Abundance in Liver Tissue of Overfeeding Goose
摘要
Abstract
[Objective] An experiment was conducted to investigate different fatty acids deposition, FAS mRNA expression abundance and their correlation in goose liver tissue in different overfeeding periods. [Method] Two hundred Qingnong gray geese at the age of 100 days were selected and overfed. Ten geese were randomly selected and slaughtered every 6 days. The weight of liver, EE, the content of fatty acids and FAS mRNA expression abundance were measured. [Result] The weight and the fat content of liver increased with the extension of overfeeding time. They arrived to the peak during 18-24 d (504.67 g/6 d) and 12-18 d secondly. The content of SFA and MUFA in liver increased with the extension of overfeeding time, especially during 12-18 d and 18-24 d, however, PUFA deposited mainly in the late period (18-30 d). Overall, in the whole process of overfeeding, fatty acids in the late period deposited significantly (P<0.05) or highly significantly (P<0.01) more than the early stage. Every 100 g liver tissue has 0.6028 g myristic acid (C14 : 0), 17.72 g palmitic acid (C16 : 0), 7.25 g octadecanoic acid (C18 : 0), 2.75 g palmitoleic acid (C16 :1), 37.42 g oleic acid (C18 : 1), 0.3078 g dodecenoicacid (C20 : 1), 0.43 g linoleic acid (C18 : 2) which were mainly deposited after overfeeding 30 days. FAS mRNA expression abundance increased to the peak at the overfeeding 24th day, then decreased quickly. During overfeeding 0-24 d, FAS mRNA expression abundance in goose liver tissue had a highly significant positive correlation with the fatty liver weight, EE, SFA and MUFA (P<0.01), and a negative correlation with PUFA although it was not significant (P>0.05). At the overfeeding 30th day there was no significant correlation between them (P>0.05).[Conclusion] The research showed that the content of EE, SFA and MUFA in liver increased with the overfeeding time and the fatty liver weight. The deposition velocity was the fastest in the period of overfeeding 18-24 d. In mRNA expression, FAS gene regulated the deposition velocity of the fat of liver tissue in fatty liver goose. It increased rapidly in the early and middle overfeeding period, however decreased in the late period.关键词
填饲鹅/脬脏/脂肪酸/FAS基因Key words
overfeeding goose/ liver/ fatty acids/ FAS gene引用本文复制引用
舒常平,王宝维,李桢,葛文华,张名爱,岳斌..填饲鹅肝脏组织中脂肪酸沉积与FAS基因mRNA的表达丰度[J].中国农业科学,2012,45(10):2002-2011,10.基金项目
国家水禽产业技术体系专项(CARS-43-11)、山东省农业良种工程资助项目(2010LZ015-04-2) （CARS-43-11）
