中国农业科学2012，Vol.45Issue(10)：2052-2057,6.DOI:10.3864/j.issn.0578-1752.2012.10.018
动物源大肠杆菌PMQR基因流行性检测
Detection of PMQR Gene in Escherichia coli Isolated from Animals
摘要
Abstract
[ Objective] The objective of the study is to investigate the prevalence of oqxAB gene and other three types of plasmid-mediated quinolone resistance ( PMQR) gene in Escherichia coli isolated from free-range farms in Guangdong province. [Method] All the strains were screened for the oqxA, oqxB, qnr, qepA, aac (6')Ib-cr genes by PCR. The susceptibility of PMQR-positive strains to 18 antimicrobial agents were determined by agar dilution method. [Results] The positive rates of qniB, qnrS, aac (6') -Ib-cr, oqxA, oqxB genes were 10.49%, 18.88%, 31.47%, 44.8%, and 48.9% respectively. No qnrA, qnrC, qnrD and qepA genes were detected in any of the isolates. oqxA and oqxB have a high positive rate, and the two genes always exsist in the same isolates. PMQR gene always shows a coexstence of two or more kinds of the plasmid genes in the same isolates. PMQR positive isolates show a high rate of resistance to 18 antimicrobial agents which was widely used in veterinary clinical medicine. [Conclusion] PMQR gene is very common in veterinary clinic. E. coli isolated from animals in Guangdong province shows a high level resistance to widely used drugs. The antibiotic sensitivity spectrum is variegated. The proportion of multi-drug-resistant strains is high.关键词
oqxAB基因/大肠埃希氏菌/质粒介导/喹诺酮/耐药基因Key words
oqxAB gene / Escherichia coli/ plasmid mediate/ quinolones/ resistance gene引用本文复制引用
庄娜,陈雪影,岳磊,廖晓萍,刘雅红..动物源大肠杆菌PMQR基因流行性检测[J].中国农业科学,2012,45(10):2052-2057,6.基金项目
国家自然科学基金-广东省政府自然科学基金联合基金重点项目(U0631006)、广东省自然科学基金创新团队项目(5200638)、教育部创新团队项目(IRT0723)和国家自然基金项目(30800836) （U0631006）
