中国农业科学2012，Vol.45Issue(11)：2260-2266,7.DOI:10.3864/j.issn.0578-1752.2012.11.015
产蛋前期和产蛋期籽鹅组织内参基因的稳定性
Stability of Endogenous Reference Genes in Green-Goose Tissues During Prelaying and Laying Periods
摘要
Abstract
[Objective] The mRNA expression levels of seven candidate reference genes in green-goose tissues during prelaying and laying periods were comparatively-analyzed. Finally the most appropriate reference genes and their numbers were screened in gene expression analysis of green-goose tissues. [ Method] The expressions of candidate reference genes 28S rRNA, 18S rRNA, GAPDH, ACTB, HPRT1, SDH and TUB in prelaying and laying green-goose liver, kidney, heart, leg muscle and ovary were detected by qRT-PCR. Gene copy number was determined using absolute quantification. Then the expression stability of these genes was analysed by geNorm software. [Result] The results showed that stability of the seven reference genes from high to low was GAPDH=HRPT1 (0.195)>TUB (0.244)>28S rRNA(0.414)>18S rRNA (0.495)>ACTB (0.541)>SDH (0.610) during prelaying period and GAPDH=28S rRNA (0.128)>TUB (0.181)>ACTB (0.192)>SDH (0.221)>HRPTl (0.316)>18S rRNA (0.362) during laying period by geNorm analysis. And V2/3 of pairwise variance analysis of 7 candidate reference genes were separately 0.084 and 0.069 during the 2 periods. The appropriate number of reference genes was 2. [ Conclusion ] These results suggest that GAPDH and HRPT1, GAPDH and 28S rRNA are suitable reference genes and could be used to normalize mRNA levels between different samples in green-goose tissues during prelaying and laying periods.关键词
籽鹅/内参基因/实时定量反转录PCR/geNorm程序/NormFinder程序Key words
green-goose/ reference gene/ qRT-PCR/ geNorm software/ NormFinder software引用本文复制引用
计红,王忠伟,郭景茹,王建发,赫荣贺,杨焕民..产蛋前期和产蛋期籽鹅组织内参基因的稳定性[J].中国农业科学,2012,45(11):2260-2266,7.基金项目
黑龙江省自然科学基金项目(ZD201116)、黑龙江省科技厅项目(GB06B204-4)、黑龙江农垦总局项目(HNKXIV-08-05a) （ZD201116）
