中国农业科学2012，Vol.45Issue(11)：2306-2314,9.DOI:10.3864/j.issn.0578-1752.2012.11.021
鲁西牛ANGPTL6基因的3个多态位点与其生长性状的关联性分析
Association Analysis of Three SNPs Within ANGPTL6 Gene with Growth Traits in Luxi Cattle
摘要
Abstract
[Objective] The purpose of this study is to reveal the genetic variation characteristics of bovine ANGPTL6 gene and find several candidate molecular markers that related to growth traits of Luxi cattle, thus for accumulating some basic information for molecular breeding of Luxi cattle breed. [Method] As investigative materials blood samples collected from 183 Luxi cows were used and sequence variants within ANGPTL6 gene were detected by DNA pool sequencing and PCR-RFLP technology. [Result] The results showed that three novel SNPs within the intron region of ANGPTL6 gene were identified in Luxi cattle breed. x2 test showed that three loci of the Luxi cattle population were all in Hardy-Weinberg equilibrium (P>0.05). Analysis of genetic diversity indicated T2359C and G3258T loci were at intermediate polymorphic status, but C2403A locus was at low polymorphic status. Further, the linkage disequilibrium and haplotypes were analyzed among three SNPs, suggested that they are all little linked. Interestingly, haplotype of TCG (wild-type) was dominant (with frequency of 44.3%). The variance analysis showed that the different genotypes of single and combined site were all significantly or extremely significantly associated with growth traits of Luxi cattle, and the growth traits of heterozygous individuals were all higher than the homozygous individuals. [Conclusion] The three novel SNPs are all significantly related to growth traits of Luxi cattle, and the heterozygous individuals are all more superior than the homozygous individuals, which suggested these results could apply to molecular breeding industry of Luxi cattle.关键词
ANGPTL6基因/多态性/关联性/生长性状/鲁西牛Key words
ANGPTL6 gene/ polymorphism/ association/ growth trait/ Luxi cattle引用本文复制引用
李爱民,马云,杨东英,蓝贤勇,胡沈荣,雷初朝,张春雷,陈宏..鲁西牛ANGPTL6基因的3个多态位点与其生长性状的关联性分析[J].中国农业科学,2012,45(11):2306-2314,9.基金项目
国家转基因重大专项(2009ZX08009-157B,2009ZX08007-005B-07)、农业部现代肉牛牦牛产业技术体系专项(CARS-38)、国家自然科学基金(30972080,30901023,31172193)、陕西省农业科技创新项目(2012NKC01-13) （2009ZX08009-157B,2009ZX08007-005B-07）
