中国水产科学2012，Vol.19Issue(3)：500-508,9.DOI:10.3724/SP.J.1118.2012.00500
近江牡蛎HSC70基因对溶藻弧菌感染的反应
mRNA expression of heat-shock cognate protein 70 gene (HSC70) in the oyster (Crassostrea hongkongensis) in response to Vibrio alginolyticus challenge
摘要
Abstract
We developed a fluorescent real-time quantitative RT-PCR method to evaluate the pattern of HSC70 mRNA expression in response to the bacterial infection in the oyster (Crassostrea hongkongensis). We injected oysters with live Vibrio alginolyticus, and measured HSC70 gene expression in six tissues (mantle, gills, digestive gland, muscle, heart and hemocytes). We measured the accumulation and distribution of V. alginolyticus using the plate counting method. HSC70 mRNA expression was significantly up-regulated in all the six tissues following injection with V. alginolyticus, and the expression was time-dependent. HSC70 gene expression increased significantly at 6 and 72 h in the gill. Similarly, levels peaked at 6 h in the digestive gland then remained high until 24 h, after which they decreased rapidly to control levels. HSC70 mRNA expression was ~10 fold higher in the haemo-cytes than the control after 3 h, and the expression evels peaked at 6 h (~15 fold higher than the control). The expression of HSC70 was ~2.5 fold higher in the remaining tissues than in the control. The bacterial concentration was higher in the muscle than that in other tissues (digestive gland and hemolymph) at 3 h after the oysters were injected with V. alginolyticus, though levels had declined by 6 h. The accumulation of bacteria in the muscle increased at 12 h and remained high up to 72 h. The change in V. alginolyticus concentration was time-dependent in the digestive gland whereas the bacteria increased with time and peaked at 48 h in the hemolymph. We observed a positive correlation between HSC70 mRNA expression and the concentration of bacteria, most obvious in the digestive gland. Our results suggest that bacterial challenge induces the up-regulation of HSC70 mRNA expression and suggests that HSC70 plays an important role in protecting the oyster against the bacterial pathogen.关键词
近江牡蛎/HSC70基因/溶藻弧菌/基因表达/实时荧光定量PCRKey words
Crassostrea hongkongensis/ HSC70/ Vibrio alginolyticus/ gene expression/ quantitative real-time PCR分类
农业科技引用本文复制引用
刘志刚,张其中,张占会,崔淼..近江牡蛎HSC70基因对溶藻弧菌感染的反应[J].中国水产科学,2012,19(3):500-508,9.基金项目
国家自然科学基金项目(40576056,40976066,31170474) （40576056,40976066,31170474）
广东省自然科学基金项目(07300378) （07300378）
中国博士后科学基金一等资助金(2007042144). （2007042144）
