中国实验动物学报2012，Vol.20Issue(2)：32-36,5.DOI:10.3969/j.issn.1005-4847.2012.02.007
微卫星DNA标记在近交系大鼠HFJ和MIJ遗传监测中的应用
Microsatellite DNA analysis for genetic monitoring of inbred strain HFJ and MIJ rats
摘要
Abstract
Objective The polymorphism of microsatellite loci among four inbred strains of rats ( HFJ, MU, Lewis, F344) was analyzed using 24 pair primers. Methods To extract the genomic DNA of MU, HFJ, Lewis and F344 inbred rats by standard extraction procedure with phenol/chloroform. 24 microsatellite loci were selected for PCR amplification. Through native polyacrylamide gel eleclrophoresis and silver staining, microsatellite polymorphism was analyzed in the four strains of inbred rats. Results The amplified products of 24 microsatellite loci among different individual rats of these 4 strains and the same strain rats displayed single allelic gene band. Among the 24 microsatellite loci, it displayed mono-morphism between HFJ and MU rats. But HFJ and MU rats had significant deviations from Lewis and F344 rats. 14 micro-satellite loci turned out to be polymorphic, and 10 microsatellite loci displayed monomorphism. Conclusions The results of this study suggest that MU and HFJ strains basically meet the requirement of inbred strain. The 14 screened loci can be used for genetic monitoring of these inbred rats.关键词
HFJ大鼠/MIJ大鼠/微卫星DNA/遗传监测Key words
Inbred rat HFJ/ Inbred rat MU/ Inbred F334 rat/ Inbred Lewis rat/ Microsatellite loci/ Polymorphism/ Genetic monitoring分类
生物科学引用本文复制引用
郑龙,李建辉,王俊霞,刘军须,蔡月花,连伟光,刘福英..微卫星DNA标记在近交系大鼠HFJ和MIJ遗传监测中的应用[J].中国实验动物学报,2012,20(2):32-36,5.基金项目
河北省科技支撑计划重点项目(09276426D). （09276426D）
