中国实验动物学报2012，Vol.20Issue(2)：64-69,封3,7.DOI:10.3969/j.issn.1005-4847.2012.02.013
应用高脂饮食联合维生素D3建立慢性系膜增殖性肾炎血管病变大鼠模型
Establishment of a rat model of progressive mesangial proliferative glomerulonephritis with vasculopathy by high fat diet and vitamin D3
摘要
Abstract
Objective To establish a rat model of progressive mesangial proliferative glomerulonephritis with vasculopathy by high fat diet feeding. Methods Male Wistar rats were subjected to unilateral nephrectomy and the surviving rats were randomly assigned into 4 groups: high fat diet group, nephritis group, nephritis fed with high fat diet group, and unilateral nephritis rats severed as controls. OX7 (100 mg/kg) antibody was firstly injected into the tail vein of rats of the nephritis group and nephritis fed high (at diet group at 3 days after nephrectomy, and then injected once per week for 3 times. The rats of nephritis group and high fat diet group received saline injection. Vitamin D3 was injected intraperitoneal-ly in a dose of 600 000 U/kg to the rats at the second day after OX7 antibody injection, once per week for 4 weeks and fed with hgh fat diet. The general status was recorded and the body weight, systolic blood pressure, proteinuria, serum total protein, serum albumin, triglyceride, cholesterol,serum creatinine and serum calcium were measured. The renal function was assessed and renal alterations were observed by histopathology. Results Intrarenal arterial wall thickening, arterial stenosis and proliferation of fibrous tissue were observed in the rats of nephritis and high fat diet group at week 8. At week 10, the intrarenal arterial wall was slightly thickened in the rats of nephritis and high fat diet group. The scores of glomeru-lar, tubulointerstitial and vascular histological damages in the rats of nephritis with high fat diet group were significantly higher than those in the rats of nephritis group and high fat diet group (P < 0.05). Conclusions Animal model of progressive mesangial proliferative glomerulonephritis with vasculopathy can be successfully established by high fat diet plus intraperitoneal injection of vitamine D3 in chronic anti-Thyl nephritis rats.关键词
高脂/系膜增殖性肾炎/血管病变Key words
High fat diet/ Mcsangial prolifcrative glomerulonephritis/ Vasculopalhy/ Rats分类
生物科学引用本文复制引用
张承英,张建荣,耿燕秋,丁韬..应用高脂饮食联合维生素D3建立慢性系膜增殖性肾炎血管病变大鼠模型[J].中国实验动物学报,2012,20(2):64-69,封3,7.
