中国实验动物学报2012，Vol.20Issue(2)：78-82,后插7,6.DOI:10.3969/j.issn.1005.4847.2011.01.016
不同免疫状态的小鼠对禽流感病毒H5N1感染的敏感性及其机制探讨
Susceptibility of mice strains in different immune status to avian influenza virus H5N1 infection and the possible mechanism
摘要
Abstract
Objective To compare the susceptibility of different mice strains in different immune status to H5N1 vims,and investigate the possible mechanism. Methods Germ free BALB/c mice,SPF BALB/c mice,SCID mice and nude mice were challenged with 50 μL 10 TCID50 H5N1 virus, then the body weight and survival were observed daily. In addition, the virus distribution in tissues, the cytokines in the lung, and pathological changes of the lung were also monitored. Results The four different strains of mice could be infected by H5N1 virus successfully. The germ free mice were the lowest susceptible animals, with lowest virus replication in GF mice tissues and lowest cytokine level, including TNF-α, IL-12,MIP2,GATA3,IFN-α/β/γ,especially at the 7th day. Conclusions Germ free mouse is the lowest susceptible animal to H5N1 among the four mouse strains: germ-free BALB/c,SPF BALB/c,nude and SCID mice,due to low level of virus replication and low cytokine expression.关键词
禽流感病毒H5N1/易感性/病毒复制/细胞因子/小鼠品系Key words
Avian influenza virus H5N1/ Susceptibility/ Virus replication/ Cytokine/ Mouse strains分类
生物科学引用本文复制引用
占玲俊,李枫棣,吕琦,朵建英,鲍琳琳,邓巍,马春梅,许黎黎,秦川..不同免疫状态的小鼠对禽流感病毒H5N1感染的敏感性及其机制探讨[J].中国实验动物学报,2012,20(2):78-82,后插7,6.基金项目
中央级公益性科研院所基本科研业务费:DWS201105 （）
