中国实验动物学报2012，Vol.20Issue(2)：83-86,4.DOI:10.3969/j.issn.1005.4847.2012.02.017
Gfat1基因在2型糖尿病大鼠肺组织中的表达
Expression of Gfat1 in the lung tissue of type 2 diabetic rat models
刘科 1钟海潮 1钟志勇 1刘盛来 1饶子亮 1黄小琼 1邝少松1
作者信息
- 1. 广东省医学实验动物中心,佛山 528248
- 折叠
摘要
Abstract
Objective To observe the expression of glutamine fructose-6-phosphate aminotransferase t (Gfatl) in the lung tissue of type 2 diabetic rats. Methods Sprague-Dawley rats were randomly divided into control group (n = 18) and diabetic model group (n = 28). Rats in the model group were fed with high-fat diet for 2 months, and then were injected intraperitoneally streptozotocin (STZ) at a dose of 15 mg/kg to generate diabetic models. Fasting blood glucose and body weight were recorded. The expression of Gfatl mRNA in the lung tissue was detected by RT-PCR on the 2nd, 4th and sixth week after diabetes modeling, respectively. Results The body weight of rats in the model group increased rapidly. Compared with that of the control group, body weight was significantly increased at the 28th, 42nd, 56th and 70th days from the modeling started (P <0. 05). Fasting blood glucose (FBG) in the rats of model group after STZ injection was significantly higher ( 510.0), compared with that in the control group (P <0. 01 ). Gfatl expression in the rats of model group on the second week of diabetes modeling was lower than that in the control group, but the difference was not significant (P > 0. 05). Gfatl expression of rats in the model group on the fourth week of diabetes modeling was higher than that in the control group, with a significant difference ( P < 0.05 ) . Gfatl expression in the rats of model group on the sixth week of diabetes modeling was higher than that in the control group, but the difference was not significant (P > 0. 05 ). Conclusions Type 2 diabetic rat model can be successfully established by feeding high-fat diet with intraperito-neal injection of streptozotocin. The level of Gfatl expression is changed in the lung tissue at different periods of diabetes modeling.关键词
2型糖尿病模型/大鼠/Gfat1/表达/肺脏Key words
Type 2 diabetes mellitus/ Model, rats/ Gfatl/ Expression/ Lung分类
生物科学引用本文复制引用
刘科,钟海潮,钟志勇,刘盛来,饶子亮,黄小琼,邝少松..Gfat1基因在2型糖尿病大鼠肺组织中的表达[J].中国实验动物学报,2012,20(2):83-86,4.
