中国比较医学杂志2012，Vol.22Issue(4)：23-26,后插2-后插4,7.DOI:10.3969/j.issn.1671.7856.2012.04.006
唾液酸受体并非流感病毒各亚型在雪貂组织中播散分布的决定因子
Sialic Acid Receptors Are not Determinant Factors for the Organ Distribution of Different Subtype Influenza Viruses in Ferret
摘要
Abstract
Objective To investigate the relationship between tissue tropism of influenza viruses and sialic acid receptors in ferret. Methods The distribution of influenza viruses H5N1 (SZ406H, A/VN/1203/04), SH1N1, H3N2 (Brisbane/09, HK/09) in infected ferrets was analyzed by virus isolation, and the distribution of sialic acid receptors in tissues was determined by direct immunofluorescence assay. The binding of influenza virus to sialic acid receptor was detected by indirect immunofluorescenee assay. Results H5N1 (SZ) and H5M (A/VN/1203/04) viruses were detected in the liver, spleen, lung and intestine. In addition, H5N1 (A/VN/1203/04) virus was also detected in the brain. SH1N1 and H3N2(Brisbane/09, HK/09) viruses were detected in the intestine. Sialic acid receptors type I SAα2,6Cal and SAα2,3Gal were distributed in the spleen, heart, lung, intestine, and brain, type II SAα2,3Cal receptors were distributed in the spleen, heart, lung, intestine, brain, and liver. SH1N1 virus was bound to SAα2,6Gal in the tissues, while H5N1 was bound to SAα2,3Gal. Conclusions Our results indicate that H5N1 (SZ, VN) virus can be distributed in many organs in ferret. SH1N1 and H3N2(HK,BR) viruses are only distributed in the intestine, while both SAα2,3Gal and SAα2,6 Gal receptors are distributed in many organs. So that we would conclude that SA receptors are not determinant factors for organ distribution of different subtype influenza viruses in ferrets.关键词
流感病毒/雪貂/组织分布/唾液酸受体Key words
Influenza virus/ Ferret/ Distribution/ Sialic acid receptor分类
医药卫生引用本文复制引用
占玲俊,邓巍,鲍琳琳,吕琦,李枫棣,马春梅,许黎黎,秦川..唾液酸受体并非流感病毒各亚型在雪貂组织中播散分布的决定因子[J].中国比较医学杂志,2012,22(4):23-26,后插2-后插4,7.基金项目
中央级公益性科研院所基本科研业务费:DWS201105. （）
