中国比较医学杂志2012，Vol.22Issue(4)：30-35,6.DOI:10.3969/j.issn.1671.7856.2012.04.008
云南地区恒河猴线粒体DNA控制区遗传多态性研究
Genetic Polymorphism of Mitochondrial DNA Control Region in Macaca mulatta from Yunnan Province
摘要
Abstract
Objective To analyze the genetic diversity and phylogenetic of Macaca mulatta in Yunnan Province, which provide important reference and background data for researches of macaca mulatta protection and reasonable application. Methods Ninety six blood samples of Macaca mulatta were collected for amplifying the complete sequences of mitochondrial DNA control region by polymerase chain reaction (PCR). The variation sites, parsimony informative sites, haplotypes, haplotype diversity, and nucleotide diversity were analyzed using Mega 4.0 and DNA SP software. The phylogenetic tree was constructed by neighbor-joining (NJ) and minimum-evolution( ME) methods. Results One hundred and forty-nine variation sites and 46 haplotypes were found in the ninety-sixth Macaca mulatta, the haplotype diversity ( Hd) was 0. 968 ± 0. 007 and nucleotide diversity ( Pi) was 0. 020. The phylogenetic analysis based on mitochondrial control region showed that these Macaca mulatto lived in Yunnan was divided into two sister groups geographically. Conclusions The results of this study indicate a high variation and phylogenetic evolution relationship of Macaca mulatta in Yunnan province.关键词
恒河猴/线粒体DNA/单倍型/遗传多态性Key words
Macaca mulatta/ mtDNA/ Haplotype/ Genetic polymorphism分类
医药卫生引用本文复制引用
禹文海,黄芬,杨凤梅,鲁帅尧,赵远,王俊斌,陈丽雄,李艳艳,和占龙..云南地区恒河猴线粒体DNA控制区遗传多态性研究[J].中国比较医学杂志,2012,22(4):30-35,6.基金项目
云南省应用基础研究面上项目(编号:2008ZC069M,2009ZC184M) （编号:2008ZC069M,2009ZC184M）
云南省技术创新人才培养基金(2009CI117). （2009CI117）
