中国比较医学杂志2012，Vol.22Issue(4)：53-57,后插6,6.DOI:10.3969/j.issn.1671.7856.2012.04.013
高热量高蛋白饮食诱导GK大鼠糖尿病肾病模型的建立
Establishment of a GK Rat Model of Diabetic Nephropathy Induced by High-Calorie and High-Protein Diet
摘要
Abstract
Objective To establish a CK rat model of type 2 diabetic nephropathv induced by high-calorie and high-protein diet, and to explore its possible mechanism of action. Methods A total of 24 28-week old CK rats were randomly divided into normal group and model group, 12 in each group. The normal rats were given normal diet while the model rats were fed a high-calorie and high-protein diet for 8 weeks. 24 h-U-ALB, 24 h-U-TP, Ucr, U-ALB/Ucr were determined at week 0, 4, and 8. FBG, Scr, BUN, TC, TG, NO were measured at week 4 and 8. The rats were sacrificed 8 weeks later, and the two kidneys of each rat were taken and weighed to calculate the kidney hypertrophy index. The renal pathological changes were examined and the Na+ K+ -ATPase activity in renal tissue was detected. Results The 24 h-U-ALB, 24 h-U-TP, U-ALB/Ucr, FBG, TC, TG, NO, kidney hypertrophy index and Na+ K+-ATPase activity were significantly increased in the model group rats. They showed an increased glomerular volume of the kidney, mesangial matrix hyperplasia and thickening of the glomerular basement membrane. Conclusions A GK rat model of type 2 diabetic nephropathy is successfully established by a high-calorie and high-protein diet. The rise in blood glucose and lipid levels contribute to the development of diabetic nephropathy. Besides, increased activity of Na + K + -ATPase further damages the function of renal tubules. The increased NO leads to glomerular hyperperfusion and hyperfiltration. These factors also accelerate the formation of nephropathy in GK rats.关键词
2型糖尿病肾病/GK大鼠/高热量高蛋白饮食Key words
Type 2 diabetic nephropathy/ GK rats/ High-calorie and high-protein diets分类
医药卫生引用本文复制引用
徐孝平,寿旗扬,陈方明,周卫民,蔡月琴,陈民利..高热量高蛋白饮食诱导GK大鼠糖尿病肾病模型的建立[J].中国比较医学杂志,2012,22(4):53-57,后插6,6.基金项目
浙江省科技厅实验动物计划项目(编号:2009F80021). （编号:2009F80021）
