中国比较医学杂志2012，Vol.22Issue(5)：31-36,后插7-后插8,8.DOI:10.3969/j.issn.1671.7856.2012.005.008
C4亚型人肠道病毒71型的小鼠肌肉适应株对小鼠具有致死毒力
A Mouse-muscle-adapted Human Enterovirus 71 Strain of C4 Subgenotype Causes Lethal Symptoms in Mice
摘要
Abstract
Objective To establish the mouse model for application in the study of pathogenesis of EV71 infection and evaluation of vaccines or drugs. Methods A mouse-adapted strain MP10 was generated by a series of passages of the parental EV71 strain in the skeletal muscle of mice. And then the symptoms, virus replication and pathological changes of tissues of infected-mice were monitored. Results Compared to the parental strain, MP10 showed enhanced infectivity in RD cells and virulence in mice, and MP10 infection caused paralysis and death in 10-day-old mice. Skeletal muscle was the main site for virus replication and showed extensive necrotizing myositis, whereas no lesions were observed in neurological tissues. The muscles associated with respirometric activity showed severe necrosis, which might have caused the infected mice to experience difficulty in breath and was thought to be the cause of death. Conclusion The 10-day-old mouse model for lethal EV71 infection was established and it would be useful for vaccine and drug evaluation.关键词
人肠道病毒71型/肌肉适应株/坏死性肌炎/模型/小鼠Key words
Human enterovirus 71/ Muscle-adaptation/ Necrotizing myositis/ Model/ Mouse分类
医药卫生引用本文复制引用
马春梅,刘江宁..C4亚型人肠道病毒71型的小鼠肌肉适应株对小鼠具有致死毒力[J].中国比较医学杂志,2012,22(5):31-36,后插7-后插8,8.基金项目
中央级公益性科研院所基本科研业务费(DWS200901). （DWS200901）
