中国比较医学杂志2012，Vol.22Issue(5)：44-50,后插9,8.DOI:10.3969/j.issn.1671.7856.2012.005.010
北京地区实验动物绿脓杆菌分离株MLVA分型
Multiple-locus Variable-number Tandem-repeat Analysis for Genotyping of Pseudomonas aeruginosa in Beijing
邢进 1吴军 2岳秉飞 1贺争鸣2
作者信息
- 1. 军事医学科学院生物工程研究所,北京100071
- 2. 中国食品药品检定研究院实验动物资源研究所,北京100050
- 折叠
摘要
Abstract
Objective To identify genotypes and potential routes of dissemination of P. aeruginosa isolates in Beijing using Multilocus-locus variable-number tandem-repeat(VNTR) analysis ( MLVA). Methods One hundred and forty one experimental animals and facilities isolates of P. aeruginosa were detected by selected 13 VNTRs loci. Then we draw the phylogenetic tree and minimum spanning tree( MST) using BioNumerics software according to fingerprint clustering analysis. Results All isolates of P. aeruginosa was divided into three major genetic Clusters, 56 genotypes. The proportion of A cluster is 82.3% , B cluster 12. 8% , C cluster 5.0% . The Simpson's index of diversity is 0. 763. The isolates have distant homology in the neighboring facilities. Conclusions MLVA displayed excellent discriminatory power for P. aeruginosa. MLVA can effectively track P. aeruginosa source. The P. aeruginosa isolates of experimental animals have rich polymorphism genotypes and no regional homology in Beijing.关键词
绿脓杆菌/多位点可变数目串联重复序列/基因分型/实验动物Key words
Pseudomonas aeruginosa/ MLVA/ Genotyping/ Experimental animal分类
医药卫生引用本文复制引用
邢进,吴军,岳秉飞,贺争鸣..北京地区实验动物绿脓杆菌分离株MLVA分型[J].中国比较医学杂志,2012,22(5):44-50,后插9,8.
