中国比较医学杂志2012，Vol.22Issue(5)：51-55,5.DOI:10.3969/j.issn.1671.7856.2012.005.011
药物安全性评价中豚鼠全身过敏试验方法的研究
Research on the Methods of Active Systemic Anaphylaxis on Guinea Pig in Drug Safety Evaluation
摘要
Abstract
Objective To analyze some potential factors which might affect the active systemic anaphylaxis (ASA) test on guinea pig. Methods In GLP conditions, guinea pigs from 300 to 400 grams were used as experimental subjects. By using the materials including bovine serum albumin and saline which are recommended for the control subjects by the guidelines, some experimental design factors such as sensitizing dose, challenge dose, challenge interval and challenge injection route, and the frequency of challenge were studied during the systemic anaphylaxis in guinea pigs. Results In a certain dose range, the incidence of allergic reactions in guinea pigs was not related with systemic sensitizing dose, challenge dose and challenge injection approach, but the severity of anaphylaxis symptoms has an obvious relationship with sensitizing dose, challenge dose and challenge injection route. Conclusion Study on the drug-induced anaphylaxis should refer to the guidelines and pay more attention to experimental design dose and the route of administration.关键词
豚鼠/致敏/激发/全身过敏试验Key words
Guinea pig (Cavia porcellus) /Sensitization/Challenge/ASA (active systemic anaphylaxis)分类
生物科学引用本文复制引用
翁谢川,丁日高,樊星,王青秀,施畅,李磊宁,欧阳兆和,孔琦,王全军,关勇彪..药物安全性评价中豚鼠全身过敏试验方法的研究[J].中国比较医学杂志,2012,22(5):51-55,5.基金项目
国家自然科学基金资助项目(No.30500620) （No.30500620）
重大新药创新专题(2008Zx09305-003) （2008Zx09305-003）
北京市自然科学基金资助项目(7092079). （7092079）
