中国兽医科学2012,Vol.42Issue(6):557-561,5.
貂源铜绿假单孢菌的分离鉴定及其致病性研究
Isolation and identification of Pseudomonas aeruginosa from minks and study on pathogenicity of P. aeruginosa
摘要
Abstract
A Pseudomonas aeruginosa strain was isolated from the lung of a dead mink from Weihai of Shandong Province. We identified the P. aeruginosa based on colony characteristics, biochemical tests and PCR. Pathogenicity test showed that the LD50 for mice was 1.4× 107 CFU/mL,and the MLD was 8.0× 106 CFU/mL. we successfully isolated the same bacteria as infectious bacteria. Drug susceptibility test showed that the isolated strain was sensitive to drugs including gentamicin, ciproflaxin, norfloxacin, and cefoperazone,but resisted to penicillin and streptomycin. Phylogenetic analysis showed that the isolate was closely related to members of P. aeruginosa from the wheat, therefore we inferred the bacteria originally coming from contaminated feed.关键词
铜绿假单孢菌/分离鉴定/致病性/药敏试验Key words
Pseudomonas aeruginosa/isolation and identification/pathogenicity/drug susceptibility test分类
农业科技引用本文复制引用
孙见,谭斌,温永俊,张淑琴,胡嘉欣,师新川,武华..貂源铜绿假单孢菌的分离鉴定及其致病性研究[J].中国兽医科学,2012,42(6):557-561,5.基金项目
“十一五”国家科技支撑计划项目 ()
公益性行业(农业)科研项目 ()