中国体外循环杂志2012，Vol.10Issue(2)：114-119,6.
深低温停循环大脑海马区差异性miRNAs表达变化及机制探讨
Identification and modulation of microRNA in hippocampus during deep hypothermic circulatory arrest
王小华 1吉冰洋 1孙燕华 1朱贤 1陈燕 1王世磊 1刘晋萍 1龙村1
作者信息
- 1. 100037,北京,中国医学科学院,北京协和医学院,阜外心血管病医院体外循环科
- 折叠
摘要
Abstract
Objective The cerebral injury after deep hypothermia and circulatory arrest ( DHCA ) is serious problem and a lew is known about its molecular mechanism. In this study, we constructed the DHCA piglet model to investigate expression profile of miRNAs associated with DHCA brain injury. Methods Six male piglets ( 2. 0 — 2. 5 kg ) were randomly divided into the DHCA group and the Sham group, hippocampus were harvested for miRNA microarray analysis. All microarray data were analyzed by Significance A-nalysis of Microarrays ( SAM ), and this result was further confirmed by qRT — PCR assay, biological information analysis to predict the target of the panels of miRNAs, then the GO analysis to find the function of the target by MAS. Results Thirty - five miRNAs were differentially expressed in hippocampus after DHCA procedure. Thirteen miRNAs ( miR —23a, miR —27a, miR — 182, et al ) were significantly up — regulated and twenty —two miRNAs ( miR — 10b, miR —200c, miR —210, miR — 150, et al ) were down — regulated in DHCA hippocampus. Bioinformatic analysis of the predicted targets for this panel of miRNAs revealed multiple protein targets, biological processes and functions involved in pathphysiology of DHCA cerebral injury including signal transduction, transcriptional regulation, apoptosis, and inflammatory. Conclusion DHCA cerebral injury involves a set of miRNAs dysregulated. MiR — 10b, miR — 200c, miR —210, miR — 150, miR —23a, et al play important role in regulate cerebral injury.关键词
深低温停循环/脑损伤/microRNA/基因芯片/生物信息学分析Key words
Deep hypothermic circulatory arrest/ Cerebral injury/ MicroRNAs/ Bioinformatics analysis分类
医药卫生引用本文复制引用
王小华,吉冰洋,孙燕华,朱贤,陈燕,王世磊,刘晋萍,龙村..深低温停循环大脑海马区差异性miRNAs表达变化及机制探讨[J].中国体外循环杂志,2012,10(2):114-119,6.
