中国畜牧兽医2012，Vol.39Issue(4)：31-34,4.
绒山羊USP9Y基因的BAC筛选与鉴定
Screening and Identification of Cashmere Goats USP9Y Gene
摘要
Abstract
USP9Y gene is closely associated with sperm production, and it plays a very important role in animal breeding. Using cow USP9Y gene sequence included in NCBI as primer design,USP9Y gene was screened by PCR technology in Cashmere goats BAC library, cloned and determined in this study to further investigate the mechanism of goats sperm production and improve the breeding rate. We compared the obtained fragment with the sequences of other species in NCBI. The alignment results showed that the homologies with cattle and felwa cat respectively were 98% and 95%, the homology with Homo sapiens ,Macaca mulatto ,chimpanzees and other species was 89%. It indicated that USP9Y gene sequences had a certain conservative in the evolutionary process. The study will lay a basis to further gain the total sequence of USP9Y gene through sequencing BAC,research gene function and fully develop the economic value of Cashmere goats.关键词
绒山羊/USP9Y/BAC/筛选/鉴定Key words
Cashmere goats/USPSY/BAC/screening/identification分类
农业科技引用本文复制引用
肖红梅,刘志红,张文广,李金泉..绒山羊USP9Y基因的BAC筛选与鉴定[J].中国畜牧兽医,2012,39(4):31-34,4.基金项目
国家自然基金项目(31072005):山羊Y染色体序列组装及其功能研究. （31072005）
