中国畜牧兽医2012，Vol.39Issue(4)：82-86,5.
两株猪繁殖与呼吸征病毒的分离鉴定及遗传变异分析
Isolation and Identification of Two Strains of Porcine Reproductive and Respiratory Syndrome Virus and Genetic Variation Analysis
摘要
Abstract
Two strains of porcine reproductive and respiratory syndrome virus were isolated from tissue and serum from some Guangdong pig farms and shown PRRS positive in RT-PCR testing by Marc-145. The two strains of virus can passage stably and cause typical cytopathic effecl, and fluorescence signal was detected by IFA, were named ZH-GD strain and ZS-GD strain. The analysis of variable region sequences of ORF5 and Nsp2 of two strains of virus showed that, PRRSV ZH-GD strain and ZS-GD strain were far to Europe strain LV.the homology of nuclear nucleotide sequence were 50. 9% and 49. 2%, and the homology with VR-2332 strain were 88.6% and 88.1%, with classic American CH-la strain were 94. 4 % and 93.0%. And there were 30 araino acids deletion in Nsp2. It shared the same deletion with JXA1, XH-GD and other highly pathogenic variant. Thus, the two strains of PRRSV belonged to variants of American type.关键词
PRRSV/分离鉴定/序列测定和分析Key words
PRRSV /isolation and identification/sequencing and analysis分类
生物科学引用本文复制引用
张超轶,张桂红,吴欣伟,闫明菲,长亮权,郭泗虎,黄昌力,黄震,张民泽,赵付荣..两株猪繁殖与呼吸征病毒的分离鉴定及遗传变异分析[J].中国畜牧兽医,2012,39(4):82-86,5.基金项目
国家自然科学基金会(NSFC)-广东联合基金项目(U0931003) （NSFC）
国家生猪现代农业产业技术体系(NYCYTX009). （NYCYTX009）
