中国畜牧兽医2012，Vol.39Issue(4)：233-237,5.
高效液相色谱—紫外法对13种磺胺类药物的同步检测
Simultaneous Determination of 13 Sulphonamides by High Performance Liquid Chromatography-Ultraviolet (HPLC-UV)
摘要
Abstract
To establish a high performance liquid chromatography-ultraviolet (HPLC-UV) method for determination of several kinds of sulphonamides residues. By the analysis of the ultraviolet wavelength scanning to 13 kinds of sulfa drugs such as SD, SA, SQ, SUL, SMM, SMD, SDM', SIZ, ST, PST, SMZ, SST and ST-Na, 270 nm is used for detection wavelength. Due to the differences of pH value and structure among drugs, a variety of combinations methods were compared. Acetonitrile and acetate were used as mobile phase for gradient elution, which can separate 13 sulphonamides. There were 10 chromato-graphic peaks, three of ST-Na, PST and ST overlapped, and additionally two of SDM'and SMZ overlapped. The method to extract samples by acetonitrile-chloroform was proved to be good because of less interferences from impurities, over 80% recovery rate and faster analytical time.关键词
磺胺类药物/高效液相色谱法/同步/检测Key words
sulphonamides/chromatography-ultraviolet/simultaneous/determination分类
农业科技引用本文复制引用
傅宏庆,王颖,张丹,王捍东..高效液相色谱—紫外法对13种磺胺类药物的同步检测[J].中国畜牧兽医,2012,39(4):233-237,5.基金项目
江苏省优势学科和江苏省高校重点实验室开放课题资助. （）
