中国畜牧兽医2012，Vol.39Issue(6)：31-37,7.
角蛋白26基因在超细型和细型细毛羊皮肤组织中的表达差异
Expression Difference of Keratin 26 Gene of Skin Tissues in Superfine and Fine Merino Sheep
摘要
Abstract
In this study, a Real-time PCR system was developed for detection of Keratin 26 (KRT26) gene expression difference of skin tissues in Superfine and fine Merino sheep using 18S rRNA.$-actin and GAPDH genes as internal control genes based on SYBR Green I dying technique. The results showed that threshold cycle(Ct) of the standard curve displayed good linear relationship with the concentrations of standard cDNA in a certain range. Significant difference of KRT26 expression were observed in the skin tissues of different fineness of wool sheep, and the expression ratio between super-fine and fine wool was 1. 5 when the 18S rRNA,p"-actin and GAPDH were reference genes respectively. It might be sure the relationship with KRT26 and the wool fineness, and this might be provide a foundation for molecular breeding and improvement of the wool traits of sheep.关键词
KRT26/细度/绵羊/皮肤组织/表达差异Key words
KRT26/fineness/sheepiskin tissues/expression difference分类
生物科学引用本文复制引用
田月珍,马依拉·吐尔逊,艾买提·买买提,狄江,吴伟伟,田可川,黄锡霞,徐新明,哈尼克孜·吐拉甫,付雪峰,张艳花..角蛋白26基因在超细型和细型细毛羊皮肤组织中的表达差异[J].中国畜牧兽医,2012,39(6):31-37,7.基金项目
现代农业产业技术体系建设专项“缄毛用羊产业技术体系”(CAR-40) （CAR-40）
国家“十二五”科技支撑项目“优质肉,毛羊新品系选育关键技术研究及示范”(2011BAD28B05). （2011BAD28B05）
