中国畜牧兽医2012，Vol.39Issue(6)：41-46,6.
猪圆环病毒2型TaqMan实时荧光定量PCR检测方法的建立
Establishment of TaqMan Real-time Quantitative PCR Method for Detection of PCV2
摘要
Abstract
The research with porcine circovirus type 2 (PCV2) of the ORF2 capsid protein gene for the purpose, design and synthesis of specific primers and probes. Gene was amplified by PCR and cloned into the pMD18-T vector, screened positive plasmid standards. By quantitative PCR optimization of reaction conditions, establishment of a real-time detection of PCV2 TaqMan quantitative PCR method. The results showed that the method was specificity, the pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus (PRRSV), swine fever virus (CSFV) and other common diseases of the original pig detection results were negative. Compared with ordinary PCR method, the sensitivity was 100 times higher, up to 100 cop-ies/μL; for the detection of clinical samples showed that the method can effectively detect lymph nodes, lungs and other tissues of PCV2.关键词
猪圆环病毒2型/荧光定量PCR/TaqMan探针/标准曲线Key words
porcine circovirus type 2/fluorescent quantitative PCR/TaqMan probe/standard curve分类
农业科技引用本文复制引用
曾思遥,张淑琼,余绍华,唐志玲,陈瑞爱,罗满林..猪圆环病毒2型TaqMan实时荧光定量PCR检测方法的建立[J].中国畜牧兽医,2012,39(6):41-46,6.基金项目
省部产学研结合重大项目(2011A090200117) （2011A090200117）
广东大华农科研基金(033A201005-3). （033A201005-3）
