中国畜牧兽医2012，Vol.39Issue(6)：50-53,4.
牛微小隐孢子虫表面抗原CP15基因原核表达及其表达产物的鉴定
Prokaryotic Expression and Identification of Cryptosporidium parvum Surface Antigen CP15 Gene in Escherichia coli BL21
摘要
Abstract
To construct a recombinant plasmid contain CP15 gene of Cryptosporidium parvumiC. parvum) and obtain re-corabinant protein. The surface antigen CP15 gene fragment of C. parvum was amplified from plasmid pMD-CP15 by PCR,and subcloned into pGEX 4T-3. The fusion express recombinant vector pGEX-CP15 was constructed in E. coli BL21. The recombinant protein was induced by 1 mmol/L IPTG and identified by SDS-PAGE and Western blotting. The CP15 gene fragment was amplified correctly as the size of gene was about 390 bp, and the recombinant plasmid pGEX-CP15 was constructed. The protein band with a molecular weight of 42 ku was detected on SDS-PAGE, which totally same with the theoretical size. The expressed protein was identified by Western blotting performed with GST serum. The fusion protein of CP15 was highly expressed in E. coli.关键词
牛微小隐孢子虫/CP15基因/原核表达/鉴定Key words
Cryptosporidium parvum /CP15 gene/ prokaryotic expression/ identify分类
生物科学引用本文复制引用
满达,兰丽,王艳霞,王敏,格日勒图..牛微小隐孢子虫表面抗原CP15基因原核表达及其表达产物的鉴定[J].中国畜牧兽医,2012,39(6):50-53,4.基金项目
国家自然基金项目(30960279) （30960279）
内蒙古自然基金项目(2011BS0408). （2011BS0408）
