中国畜牧兽医2012，Vol.39Issue(6)：53-56,4.
犬瘟热病毒贵州株(CDV-GZ1)F基因的克隆及序列分析
Cloning and Sequence Analysis of Fusion Protein Gene of Canine Distemper Virus Isolated from Guizhou
摘要
Abstract
Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank, and the F gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from CDV-GZ1 strain. The amplified fragment was cloned and analyzed. The results showed that the length of CDV-GZ1 F gene was 1989 bp. It contained five potential N-glycosylation locations based on the derived amino acid sequences. The homology of coding amino acid between this strain and American 00-2601 and domestic HL strain was 91. 6% and 91. 1% respectively, it showed that they had common ancestor.关键词
犬瘟热病毒/CDV-GZ1/F蛋白/克隆/序列分析Key words
canine distemper virus/CDV-GZ1/fusion protein/cloning/sequence analysis分类
生物科学引用本文复制引用
曾智勇,周莉,刘志杰..犬瘟热病毒贵州株(CDV-GZ1)F基因的克隆及序列分析[J].中国畜牧兽医,2012,39(6):53-56,4.基金项目
贵州大学博士基金“犬瘟热病毒贵州株的分离鉴定及主要抗原蛋白编码基因的研究”(X060054). （X060054）
