中国畜牧兽医2012，Vol.39Issue(6)：79-82,4.
TaqMan探针荧光RT-PCR检测狂犬病病毒
Detection of Rabies Virus by TaqMan Probe Real-time RT-PCR
摘要
Abstract
To establish the method of TaqMan probe real-time RT-PCR for detection of rabies virus, a pair of primers and probe were designed based on the nucleoprotein sequences of rabies virus that published in GenBank. RNA was extracted from rabies virus vaccine, and then amplified by RT-PCR, cloned the target fragment and took the recombination plasmid as standard positive control. The results showed that the method can distinguished rabies virus from canine distemper virus, canine parvovirus, canine adenoviruses, canine coronavirus and canine para influenza virus, the sensitivity could attained 10 copies/μL and the stability test was good.关键词
狂犬病病毒/TaqMan探针/荧光RT-PCRKey words
rabies virus/ TaqMan probe/ real-time RT-PCR分类
农业科技引用本文复制引用
王振全,罗宝正,薄清如,周昌芳,白鸽,许远靖,王冲,吴殿君..TaqMan探针荧光RT-PCR检测狂犬病病毒[J].中国畜牧兽医,2012,39(6):79-82,4.基金项目
广东省科技计划项目(2009B030801075). （2009B030801075）
