中国畜牧兽医2012，Vol.39Issue(6)：173-175,3.
抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究
Study and Application the Hyperimmunized Yolk Antibodies of TGEV and PEDV in Piglets
摘要
Abstract
The hyperimmunized yolk antibodies against porcine transmissible gastroenteritis virus (TGEV) and poricine epidemic diarrhoea virus (PEDV) was obtained) and its cure effect was tested. The hens were immunized with the two combined inactivated vaccine of TGEV and PEDV. After the antibody titer reached to 1: 64 tested by double diffusion and eggs of hyper-immunity were collected. The hyperimmunized yolk antibodies were purified by chloroform extraction and salt precipitation with ammonium sulfate. Microorganism detection, safe experiment, artificially infected piglets with the antigen and clinical treatment test were conducted to evaluate the application effect. The cure rate of artificial infection was 100% and the cure rate of clinic treatment test was 88. 0%. The antibody had obvious curative effect against TGEV and PEDV.关键词
猪传染性胃肠炎/猪流行性腹泻/卵黄抗体/仔猪腹泻Key words
TGEV/ PEDV/ hyperimmunized yolk antibodies/piglet diarrhea分类
农业科技引用本文复制引用
崔焕忠,姜海龙,张加力,张辉,秦贵信..抗猪传染性胃肠炎病毒与猪流行性腹泻病毒卵黄抗体制备及其临床应用研究[J].中国畜牧兽医,2012,39(6):173-175,3.基金项目
吉林省重大科技攻关项目(09ZDGG008). （09ZDGG008）
