猪流感病毒实时荧光定量RT-PCR检测方法的建立OA北大核心CSTPCD
Real-Time Quantitative RT-PCR Assay for Detection of Swine Influenza Virus
本研究选取猪流感病毒(swine influenza virus,SIV)的NP基因序列设计引物和探针,建立了检测SIV的TaqMan实时荧光定量PCR方法.以梯度稀释的含有SIV目的扩增片段的质粒作为标准品,进行定量PCR反应以确定检测灵敏度.2.0×108至2.0× 102拷贝/μL 7个数量级的范围内定量PCR有“S”型扩增曲线,检测灵敏度为20个拷贝/μL.根据病毒拷贝数与Ct值的关系绘制了标准曲线.该方法具有特异性,对猪瘟病毒、伪狂犬病…查看全部>>
A real-time quantitative RT-PCR assay was developed for detection of Swine influenza virus (SIV). Primers and probe were designed based on NP gene of SIV by Primer Express 2. 0 software. The plasmid containing the target sequence was constructed to detect the sensitivity and prepare the standard curve. The real-time RT-PCR assay had a detection limit of 20 copies, with a dynamic range of detection between 2×108 to 2×102 copies/μL. The standard curve was prep…查看全部>>
张太翔;凌宗帅;岳志芹;徐彪;梁成珠;刘文鹏;张焕海;张艺兵
潍坊出入境检验检疫局,山东潍坊261041济南出入境检验检疫局,山东济南250014山东检验检疫局,山东青岛250023山东检验检疫局,山东青岛250023山东检验检疫局,山东青岛250023潍坊出入境检验检疫局,山东潍坊261041潍坊出入境检验检疫局,山东潍坊261041潍坊出入境检验检疫局,山东潍坊261041
农业科技
猪流感病毒实时荧光定量PCRTaqMan探针
SIV real-time quantitative RT-PCRTaqMan probe
《中国畜牧兽医》 2012 (6)
181-184,4
国家质量监督和检验检疫总局科研立项(2010IK023).
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