一株鸡传染性法氏囊病病毒的分离鉴定OA北大核心CSTPCD
Isolation and Identification of One Strain Infectious Bursal Disease Virus
从天津地区免疫失败的鸡群中分离一株IBDV(命名TJ-hg),应用血清学AGP试验结果可见明显的白色沉淀线；应用逆转录酶—聚合酶链式反应( RT- PCR)扩增的IBDV VP2基因高变区(vVP2)及其序列分析的结果表明,TJ-hg株与超强毒株的核苷酸同源性均在90％以上,氨基酸同源性达98.8％～99.3％,且高变区中特征性氨基酸的变化与超强毒株完全一致,TJ-hg株在第一亲水区氨基酸Q219P发生突变.研究结果表明,TJ-hg株属于超强毒株…查看全部>>
One isolate of infectious bursal disease virus (IBDV) ,was isolated from the vaccination failure chicken. Through serologic AGP experiment,results showed obvious white precipitation linei the high variable (hv) region of VPZ genes were amplified by RT-PCR (reverse transcriptase polymerase chain reaction) and were compared for their sequences. It was indicated that the homology of TJ-hg strain and very virulent IBDV strain all reached above 90% and 98. 8% to …查看全部>>
赵云英;张燕欣;张文生;李富桂
天津农学院动物科学系,天津300384天津农学院动物科学系,天津300384天津职业大学生物与环境工程学院,天津300402天津农学院动物科学系,天津300384
农业科技
IBDV超强毒株分子鉴定
IBDV very virulent strain molecular identification
《中国畜牧兽医》 2012 (6)
207-211,5
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