中国畜牧兽医2012，Vol.39Issue(6)：212-214,3.
一株猪捷申病毒的分离与鉴定
Isolation and Identification of Porcine Teschovirus
王美君 1宫晓文 1张志榜 1倪娇 1焦连国 1赵亚荣1
作者信息
- 1. 北京大北农动物医学研究中心,北京100097
- 折叠
摘要
Abstract
Some pig groups doubtfullyy infected on porcine teschovirus (PTV) in Beijing area. In order to diagnose this virus's infection, isolated the virus from the brain organization sample, and withdrawed total RNA from the separation virus, according to reported sequence. After using RT-PCR, obtained the part code primary structure protein VP\ gene and highly conservative 5'UTR area gene order, cloned these genes to the pEASY-Blunt vector. The result indicated that the isolated virus and the porcine teschovirus homology is 95% to 100% after compared with GenBank on virus sequence. This experiment successfully isolated a porcine teschovirus by naming as 10BJ02 in Beijing area.关键词
猪捷申病毒/分离/鉴定/RT-PCRKey words
porcine teschovirus/ isolation/ identification/ RT-PCR分类
农业科技引用本文复制引用
王美君,宫晓文,张志榜,倪娇,焦连国,赵亚荣..一株猪捷申病毒的分离与鉴定[J].中国畜牧兽医,2012,39(6):212-214,3.
