摘要
Abstract
To investigate the infection status and the immune effect of the highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) from immunized pig populations of Panjin city in 2011, 2160 serum samples and 120 tissue specimens were collected from pig farms in Panjin city from January to December in 2011, and examined by enzyme linked im-munosorbent assay (ELISA) and quantitative real-time PCR respectively. The results showed that there were not the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-RRSV) in the tissues, the antibodies positive rate in Da-wa,Panshan, Xinglongtai,Shuangtaiziwas82.2%, 81.1%,78. 8% ,81, 1%, respectively; the antibodies positive rate in swine breeding farm,commercial pig farm,peasant household pigs was 90%,81. 3%,75%, respectively. In summary, the immune status of HP-PRRS was fine, HP-PRRS did not break out in this year.关键词
高致病性猪繁殖与呼吸综合征/酶联免疫吸附试验/荧光RT-PCRKey words
HP-PRRSi ELISA/ quantitative real-time PCR分类
农业科技