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SPAG6基因多态性对种公鸡精液品质和性发育相关性状的影响

毕瑜林 陈继兰 刘冉冉 郑麦青 朱静 常国斌 赵桂苹 文杰 陈国宏

中国畜牧杂志2012,Vol.48Issue(11):1-6,6.
中国畜牧杂志2012,Vol.48Issue(11):1-6,6.

SPAG6基因多态性对种公鸡精液品质和性发育相关性状的影响

Effects of Polymorphism of SPAG6 Gene on Sperm Quality and Sexual Development-related Traits in Breeder Cocks

毕瑜林 1陈继兰 2刘冉冉 2郑麦青 2朱静 2常国斌 2赵桂苹 1文杰 2陈国宏2

作者信息

  • 1. 扬州大学动物科学与技术学院,江苏扬州225009
  • 2. 中国农业科学院北京畜牧兽医研究所,农业部畜禽遗传资源与种质创新重点实验室,北京 100193
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摘要

Abstract

This study focused on the identification of SNPs in SPA G6 gene and association analysis between the selected 5 SNPs and the traits of sperm quality together with sexual development-related traits in breeder cocks. Mass SNP technology was applied to classify the genotypes of 89 male Beijing—You chickens aged 35 weeks. After identifying the haplotype with Phase and Haploview software, the linear model was constructed for association analysis. 5 SNPs of SPAG6 gene were identified, among them, C29756T and T23041C were significantly associated with semen volume, sperm motility and sperm abnormality (P<0.05); The haplotypes were significantly associated with the traits of semen volume and sperm abnormality (P<0.05); Chickens with H4H4 haplotype showed significantly higher semen volume, and lower sperm abnormality than thai with H2H3 and H3H3 haplotypes, indicating that H4H4 was the optimal haplotype for sperm quality. Collectively, these results imply that SPA G6 gene probably involved in the regulation of semen quality in chickens and the SNPs of C29756T and T23041C along with H4H4 haplotype have great potential to be used as molecular marker for selection of chickens with high-quality semen.

关键词

SPAG6/多态位点/精液品质/性发育相关性状/北京油鸡

Key words

SPA G6/ SNP/ sperm quality/ sexual development-related traits/ Beijing-You chicken

分类

农业科技

引用本文复制引用

毕瑜林,陈继兰,刘冉冉,郑麦青,朱静,常国斌,赵桂苹,文杰,陈国宏..SPAG6基因多态性对种公鸡精液品质和性发育相关性状的影响[J].中国畜牧杂志,2012,48(11):1-6,6.

基金项目

鸡分子与细胞育种新技术创新及新品种(品系)培育(2008AA101009、2011AA100305) (品系)

中央财政支农专项资金 ()

北京市科技计划课题 ()

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