中国畜牧杂志2012,Vol.48Issue(11):1-6,6.
SPAG6基因多态性对种公鸡精液品质和性发育相关性状的影响
Effects of Polymorphism of SPAG6 Gene on Sperm Quality and Sexual Development-related Traits in Breeder Cocks
摘要
Abstract
This study focused on the identification of SNPs in SPA G6 gene and association analysis between the selected 5 SNPs and the traits of sperm quality together with sexual development-related traits in breeder cocks. Mass SNP technology was applied to classify the genotypes of 89 male Beijing—You chickens aged 35 weeks. After identifying the haplotype with Phase and Haploview software, the linear model was constructed for association analysis. 5 SNPs of SPAG6 gene were identified, among them, C29756T and T23041C were significantly associated with semen volume, sperm motility and sperm abnormality (P<0.05); The haplotypes were significantly associated with the traits of semen volume and sperm abnormality (P<0.05); Chickens with H4H4 haplotype showed significantly higher semen volume, and lower sperm abnormality than thai with H2H3 and H3H3 haplotypes, indicating that H4H4 was the optimal haplotype for sperm quality. Collectively, these results imply that SPA G6 gene probably involved in the regulation of semen quality in chickens and the SNPs of C29756T and T23041C along with H4H4 haplotype have great potential to be used as molecular marker for selection of chickens with high-quality semen.关键词
SPAG6/多态位点/精液品质/性发育相关性状/北京油鸡Key words
SPA G6/ SNP/ sperm quality/ sexual development-related traits/ Beijing-You chicken分类
农业科技引用本文复制引用
毕瑜林,陈继兰,刘冉冉,郑麦青,朱静,常国斌,赵桂苹,文杰,陈国宏..SPAG6基因多态性对种公鸡精液品质和性发育相关性状的影响[J].中国畜牧杂志,2012,48(11):1-6,6.基金项目
鸡分子与细胞育种新技术创新及新品种(品系)培育(2008AA101009、2011AA100305) (品系)
中央财政支农专项资金 ()
北京市科技计划课题 ()