中国畜牧杂志2012，Vol.48Issue(11)：21-27,7.
LiCl激活经典Wnt信号通路诱导猪骨骼肌卫星细胞向慢肌分化
Activation Canonical Wnt Signaling by LiCl Induces Porcine Skeletal Muscle Satellite Cells Differentiation into Slow Muscle
摘要
Abstract
Wnt/β-catenin signaling involved in regulating cell proliferation and differentiation to determine cell fate. Lithium chloride (LJCl) is known to activate canonical Wnt signaling by inhibiting glycogen synthetase kinase-3β and consequently stabilizing free cytosolic β-catenin. To understand the role of the Wnt/β—catenin pathway in the regulation of porcine skeletal muscle satellite differentiation, to make the skeletal muscle satellite cells for the new sources of the diseases treatment. We cultured porcine skeletal muscle satellite cells in GMEM with Wo CEE, 20% FBS, and supplied 15 mmol/L LiCl to induce myoblast differentiation. Then we detected the protein expression of Wnt/β -catenin related genes and myoblast differentiation marker genes by Western blotting and immunofluorescence. The results showed that the cells protein of p-GSK-3β, β-catenin , myosin and MYH7 were increased, p-β-catenin GSK-3β and MY-32 were decreased after LiCl treatment. Additionally, the nuclear staining of endogenousβ -catenin was also significantly increased. These result demonstrated that Wnt/β-p,atenin is a significant pathway that regulates myogenic differentiation, and induced porcine skeletal muscle satellite cells differentiation to slow muscle.关键词
卫星细胞/Wnt/β-catenin/分化/LiCl/猪Key words
satellite cells/ Wnt/β-catenin/ differentiation, LiCl/ porcine分类
农业科技引用本文复制引用
杨秋梅,刘月光,沈清武,史新娥,杨公社..LiCl激活经典Wnt信号通路诱导猪骨骼肌卫星细胞向慢肌分化[J].中国畜牧杂志,2012,48(11):21-27,7.基金项目
转基因生物新品种培育重大专项基金(2009zx08009-157B) （2009zx08009-157B）
