中国药房2012，Vol.23Issue(17)：1559-1561,3.DOI:10.6039/j.issn.1001-0408.2012.17.08
非那西丁探针法动态观测非酒精性脂肪肝大鼠发病进程中的CYP1A2活性
Dynamic Observation of CYP1A2 Activity in Nonalcoholic Fatty Liver Disease Process of Rats by Phenacetin Probe Method
摘要
Abstract
OBJECTIVE: To observe the activity of CYP450 in nonalcoholic fatty liver disease process of rats by phenacetin probe method dynamically. METHODS: Nonalcoholic fatty liver rat model was established, and probe drugs were given at Oth, 7th, 9th, 11th, 13th, 15th, 17th week and at the same time urine was collected. The metabolism index of phenacetin metabolite acetaminophen was determined to investigate the activity of CYP1A2 acetaminophen with normal rats as control. HPLC method was used. The determination was performed on Hypersil ODS column with mobile phase consisted of methanol-water (12.5:87.5) at the flow rate of 1.0 mL·min‐1. The detection wavelength was set at 254 nm. RESULTS: The metabolism of acetaminophen per unit body weight reached the peak at 9th week, which is higher than at Oth week and normal group ((15.02 ± 3.12), (10.03 ± 3.60), (9.96±4.00) mg·kg‐1), and then decreased gradually, i.e. the activity of CYP1A2 increased firstly and then decreased. CONCLUSION: This method is simple and reliable for the determination of acetaminophen in rats urine and CYP1A2 activity research.关键词
探针法/高效液相色谱法/非酒精性脂肪肝模型/大鼠/CYP1A2/非那西丁/对乙酰氨基酚Key words
Probe method/ HPLC/Nonalcoholic fatty liver model/Rats/ CYP1A2/Phenacetin/ Acetaminophen分类
医药卫生引用本文复制引用
刘莹,金涌,蒋培培,吴德敏,方慧子,周静婷,季俊虬..非那西丁探针法动态观测非酒精性脂肪肝大鼠发病进程中的CYP1A2活性[J].中国药房,2012,23(17):1559-1561,3.基金项目
本文为安徽医科大学七年制临床医学专业“早期接触科研”训练计划(2011-ZQKY-91)课题资助项目 （2011-ZQKY-91）
